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Fig. 2
Accumulation of mouse and zebrafish PrPs at newly formed cell contacts in Drosophila S2 cells.
A) Expression of the mouse PrP EGFP fusion construct (m PrP) induces cell contact formation and the subsequent accumulation of PrP at contact sites. This is not observed at fortuitous contacts formed by cells expressing a control construct lacking the major PrP domains (m PrP ΔCore). Cell-cell contacts are indicated by white arrowheads. Scale bars = 5 μm. B-D) Quantification of the number of S2 cell contacts showing accumulation of wild type (WT) and mutant constructs for mouse PrP (B), zebrafish PrP-1 (C) and zebrafish PrP-2 (D). Construct names are inserted in the graphs. Double and triple asterisks [** and ***] indicate statistical significance at p<0.01 and <0.001, respectively; one-way ANOVA test; error bars indicate SEM.