ZFIN Image: Wong et al., 2013, Fig. 5

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Figure Caption

Fig. 5

Dorsomorphin inhibits the growth of FGSCs in 3 week cultures.

(A) Addition of dorsomorphin significantly inhibits FGSC (A) colony formation and (B) cell proliferation in culture. Photomicrographs showing (C) a 3-week old FGSCs (arrows) cultured in the presence of 2 μM dorsomorphin and (D) a 3-week-old FGSC colony cultured in the absence of dorsomorphin. (E) RT-PCR analysis of RNA isolated from a 3-week FGSC culture grown in the presence or absence of dorsomorphin, whole ovaries and feeder cells alone. Dorsomorphin severely reduced the expression of germ cell specific marker genes, dazl, dnd, nanos3, vasa and piwil1. * indicates a significant difference by Student t-tests. Scale bar = 20 μm.

Acknowledgments

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