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Fig. 2
RA sensor reporters are expressed in established domains of RA responsive gene expression. A, B, K, L: Reporter expression in both the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) and Tg(β-actin:GDBD-RLBD);(UAS:mCherry) lines initiates in the spinal cord (arrows in A and K) by the tailbud (TB) stage. C, D, M, N: RA sensor reporter expression is strengthened in the anterior spinal chord by the 8-somite (s) stage (arrows in C and M). E, F, O, P: RA sensor reporter expression is maintained in the anterior spinal cord (sc) and noticeable expanded to the adjacent tissues, including the notochord (nc) and ectoderm (ec), by the 16s stage. G, H, Q, R: RA sensor reporter expression was maintained in the tissues of the mid-region (anterior trunk and spinal cord), but was now more easily observed in the somites (sm) and pronephros (pn). I, J, S, T: Both the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) and Tg(β-actin:GDBD-RLBD);(UAS:mCherry) lines initiate some expression in the brain by 36 hpf. However, we only found eye expression (arrow in I) in the Tg(β-actin:GDBD-RLBD);(UAS:EGFP) line. Views in A, C, E, G, I, K, M, O, Q, S are lateral. Views in B, D, F, H, J, L, N, P, R, T are dorsal. In all images, anterior is up.