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Fig. S5
Knockdown of Cav-1b in ZFL cells. Western blot showing efficacy of MO (morpholino) in ZFL cells. Cells were transfected with Control morpholino (MO) or Cav-1b MO to knock down the expression of Cav-1b. Samples were taken at 6 and 30 h post transfection (hpt), corresponding to the time points used to perform experiments shown in Figure 7. At 6 hpt there is a marked decrease in cav-1 protein expression compared to control cells, while at 30 hpt a slight decrease in cav-1 protein expression is still observed. Membranes were re-probed with antibody against β-actin to control for protein loading.
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