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Fig. 2 Effect of rh-insulin on meiotic maturation in zebrafish oocytes in vitro. Denuded oocytes were incubated either without (control) or with different doses of rh-insulin (0.2–1 IU/ml) for indicated time intervals (a), immunoblot analyses using anti-p34cdc2 (PSTAIRE) antibody (upper panel) and time kinetics of histone H1 kinase activation (middle panel) along with oocyte photomicrographs (lower panel) from untreated control and insulin stimulated group (b), priming (2 h) with actinomycin-D (actD, 100 nM) and cycloheximide (Chx, 100 μg/ml) on rh-insulin-induced oocyte GVBD (c). Values are mean ± SEM of three independent experiments, #P < 0.001 versus 0 h control. Data were also analyzed by one-way ANOVA and Duncan’s Multiple Range Test. Values with different superscripts (c) differ significantly from each other (P < 0.05). Scale bar <100 μm, staining with Coomassie brilliant blue R250 (CBBR250) revealed equal protein loading.
Reprinted from Molecular and Cellular Endocrinology, 374(1-2), Das, D., Khan, P.P., and Maitra, S., Participation of PI3-kinase/Akt signalling in insulin stimulation of p34cdc2 activation in zebrafish oocyte: Phosphodiesterase 3 as a potential downstream target, 46-55, Copyright (2013) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.