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Fig. S1 Zebrafish synapsin Genes Are Expressed in RBs, Related to Figure 3 (A) In situ hybridization (ISH) for zebrafish synapsins1, 2a, and 2b at 25 hpf in transverse sections. synapsin1 showed the strongest expression of the three in RBs. Scale bar 50 μm. (B) synapsin1 ISH on 17, 19, 22, and 25 hpf transverse sections (2 color ISH with islet-1 to label RBs and motor neurons in 19, 22, and 25 hpf images). synapsin1 transcript was first seen at 17 hpf in motor neuron domain (arrowhead), but not in RBs (arrow). synapsin1 and islet1 were seen in RBs from 19 hpf on (arrows). Scale bar 50 μm.
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