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Fig. S3 hSmad1WT and hSmad1MM mRNAs similarly rescue Smad5-deficient dorsalized embryos and hSmad1WT and hSmad1MM are equally stable in the injected embryos. (A) Percentage of embryos that show severely (C5), moderately (C3) and mildly (C1/C2) dorsalized, wild-type (WT) and V1 (diminished eye size and increased ventral tail fin tissue) phenotypes in control and smad5 MO-injected Tg(hsp70:chd) embryos without heat shock, also injected with either hSmad1WT mRNA (250, 350 or 450 pg) or hSmad1MM mRNA (250, 350 or 450 pg). (B) Western blot for total Smad1/5 protein in the same embryos as shown in A. Embryos were collected at 80% epiboly stage. Control and smad5 MO-injected Tg(hsp70:chd) embryos, also injected with hSmad1WT mRNA (250, 350 or 450 pg) and hSmad1MM mRNA (250, 350 or 450 pg), and either not heat shocked (noHS; upper left panel) or heatshocked at 60% epiboly (upper right), 65% epiboly (lower left) or 70% epiboly (lower right).