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Fig. 2 Gentamicin localizes to utricular hair cells.
Adult Brn3c-GFP zebrafish received an intraperitoneal injection of GTTR (30 mg/kg) and allowed to recover for 4 hours. (A) Low magnification image of the utricle. GFP-labeled hair cells (green) are located throughout the organ. The striolar region (S) and extrastriolar region (E) are highlighted. High magnification images (B, C) are single optical slices from individual z-stacks from (B) striolar and (C) extrastriolar regions. GTTR fluorescence (red) was localized to hair cells (white arrows) and supporting cells (white asterisks) throughout the sensory epitheilum. GFP fluorescence (green) is localized to the cell membrane region of hair cells. The slices are taken mid-way through the cell to show the localization patterns, thus, neither hair cell nuclei nor stereociliary bundles are visible.