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Fig. 1 Gentamicin localizes to saccular hair cells.
Adult Brn3c-GFP zebrafish received an intraperitoneal injection of Texas Red-conjugated gentamicin (GTTR; 30 mg/kg) and allowed to recover for 4 hours. (A) Low magnification image of the saccule. GFP-labeled hair cells (green) are located along the length of the organ. V = ventral, R = rostral. High magnification images (B–E) are single optical slices from individual z-stacks taken at (B) 5%, (C) 25%, (D) 50%, and (E) 75% along the length of the saccule from the rostral end (see A). GTTR fluorescence (red) was localized to hair cells (D, white arrows) and supporting cells (white asterisks) throughout the sensory epithelium. GFP fluorescence (green) is localized to the cell membrane region of hair cells. The z-stack slices are taken mid-way into the cell to show the localization patterns, thus neither hair cell nuclei nor stereociliary bundles are visible.