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Fig. 4 Gentamicin treatment increases the numbers of TUNEL-labeled cells in the adult saccule.
Adult wildtype zebrafish received either a single intraperitoneal injection of (A, C, D, E) buffer or (B, F, G, H) 250 mg/kg gentamicin and allowed to recover for either 4 or 24 hours (4 hour data is shown). Fixed saccules were processed using a TUNEL-kit to label apoptotic cells (green cells; A, B, C, E, F, H) and co-labeled with DAPI (blue cells; A, B, D, E, G, H). (A-B) Low power and (C–H) high power images of the saccule reveals few TUNEL-labeled cells scattered throughout the saccule of a buffer-treated animal (A, C, E) but more TUNEL-labeling in a gentamicin-treated animal (B, F, H). (I) Mean number of TUNEL-labeled cells (± SD) in the saccules of buffer- or gentamicin-injected zebrafish. *p<0.05; n = 9–12; V = ventral, R = rostral.