ZFIN Image: Zhang et al., 2013, Fig. 2

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Fig. 2

The Arrest of Thymocyte Development in rpl22, but Not rpl22l1, Morphants Is p53-Dependent (A–D2) Knockdown of both Rpl22 and Rpl22l1 arrest T cell development, but the arrest in rpl22l1 morphants is p53-independent. Rpl22 and Rpl22l1 were knocked down in p53-sufficient (A and C) or p53-deficient (p53m/m; B and D) Tg(lck:EGFP) embryos by injection of 1 ng of Rpl22 MO, Rpl22l1 MO, or MM control, after which T cell development was assessed microscopically by scoring for the loss of GFP-marked T cell progenitors at 5 dpf (lateral view, red circles; dorsal view, yellow rectangles). Effects on apoptosis in the thymus (green dashed rectangles) were assessed at 3.5 dpf by TUNEL staining (A2, B2, C2, and D2). Images depict phenotypes representative of at least three separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure S2.

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Acknowledgments

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Reprinted from Developmental Cell, 24(4), Zhang, Y., Duc, A.C., Rao, S., Sun, X.L., Bilbee, A.N., Rhodes, M., Li, Q., Kappes, D.J., Rhodes, J., and Wiest, D.L., Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs, 411-425, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell