Mnx proteins are restricted to post-mitotic neurons. Lateral views of 12 to 14 hpf Tg(olig2:GFP) embryos. (A) Mnx2a+ cells within the spinal cord do not co-express phosphohistone H3, a marker of mitotic cells (0/153 Mnx2a+ cells in 13 embryos). Some Mnx2a+ cells strongly express GFP. (B) Mnx1+ cells within the spinal cord do not co-express phosphohistone H3 (0/70 Mnx1+ cells in 10 embryos). No Mnx1+ cells strongly express GFP. (C) Mnx2a+ cells that expressed GFP weakly or were GFP- co-expressed Elavl3, a marker of post-mitotic neurons. Mnx2a+ cells that expressed GFP strongly did not co-express Elavl3. (D) Mnx1+ cells co-expressed Elavl3. (E) Schematic of gene expression during transition from pMN progenitors to post-mitotic neurons. Mitotic progenitors express phosphohistone H3 (PH3), whereas post-mitotic neurons express Elavl3. olig2 expression is initiated in progenitors, and down-regulated as cells become post-mitotic. Both Mnx1 and Mnx2a expression is initiated after cells become postmitotic, with expression of Mnx2a preceding expression of Mnx1. Scale bar: 30 μm, A-D.
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