ZFIN Image: Sarris et al., 2012, Fig. S1

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Figure Caption

Fig. S1 zcxcl8 Is Upregulated upon Infection, and zcxcl8 Knockdown Does Not Affect Larval Development or Neutrophil Distribution (Related to Figure 1)
(A and B) qPCR analysis of zcxcl8-chr 1 (A and B), zcxcl8-chr 7a (A), zcxcl8-chr 7b (A) mRNA expression 0.5, 2 and 4 hours post injection (hpi) of E.coli. NI, non injected. Data are normalized to ef1a expression. n=3 qPCR samples. Error bars: SEM. Data in A and B are from two independent experiments, each with pools of 15-20 larvae per condition.
(C) Bright field and wide-field fluorescence images of whole Tg(mpx:gfp) larvae at 2.5 dpf treated or not (control) with splice-blocking (sMO) or combined splice and translation-blocking (sMO+tMO) zCxcl8 morpholinos. E, eye. Y, yolk sac.
(D) Flow cytometry of whole Tg(mpx:gfp) larvae, treated or not with splice-blocking (sMO) or combined splice and translation-blocking (sMO+tMO) zCxcl8 morpholinos. GFP⁺ neutrophils are boxed (green) and their percentage over the total cell number is shown. Pools of 15 larvae. Representative of four independent experiments.

Acknowledgments

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