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Fig. 5 Carbaryl-treated embryos have a reduced number of gefiltin 1 positive neurons at 24, 36, and 48 hpf. Dorsal views of whole-mount RNA in situ hybridization using gefilitin 1 on MeOH control embryos (A, C, E; n=89, 31, 53, respectively) and carbaryl-treated embryos (B, D, F; n=30, 48, 31, respectively) at 24 hpf (A and B), 36 hpf (C and D), and 48 hpf (E and F). Individual neurons are labeled. Enlarged views of panels A–F are shown (A′–F′). Quantification of gefiltin 1 positive cells indicate a statistically significant (* indicates p<0.001) decrease in the number of neurons in carbaryl-treated embryos, where error bars indicate standard deviation (G). Different focal plane images were merged in Photoshop to produce complied images. Also images (A′–F′) were sharpened in Photoshop. Scale bars: (A–F) 250 μm; (A′–F′) 50 μm.