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Fig. S1 etv5a and pea3 are expressed by the developing hypothalamus but at lower levels/in a more restricted fashion than etv5b. (A-H) Expression of etv5a and pea3 (whole-mounts). At 24 hpf, etv5a transcripts were present in the telencephalon (t), pineal/epithalamus (e), basal forebrain (bf), optic stalk (os), mid-hindbrain boundary (mhb) and rhombencephalon (rh). Progressively, the expression domain in the basal forebrain narrowed, and by 30 and 36 hpf it was present in the ventral/caudal hypothalamus (h). Expression persisted in this region at low levels until 48 hpf. pea3 was expressed in a more restricted manner in the forebrain at 24 hpf, and from 30 hpf onwards its expression in the basal forebrain was limited to a stripe at the distal limits of the hypothalamus. (I-Q) Fluorescent microscopy images of Tg(ermp:gv)3Tg(uas:gfp) transgenic embryos (whole-mount) processed for double gfp/etv5b ISH. Lateral views, anterior left. Scale bars: 50 μm.