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Figure Caption
Fig. 3 Blastema marker, msxb, and proliferation after crush injury.
(A–D) Whole-mount in situ hybridization for msxb mRNA at (A) 24hpc (B) 24hpa (C) 48hpc (D) 48hpa. Immunohistochemistry for the proliferation marker PCNA (green) at (E) 48hpc around the crush injury site and (F) 48hpa blastema (distal region is to the left and proximal to the right). DAPI (blue) is staining the nuclei. Arrowheads indicate the amputation plane and asterisks indicate crush injury sites. Scale bar corresponds to 100μm. (hpa – hours post-amputation; hpc – hours post-crush injury).
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