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Fig. 5 Knockdown of manf did not alter the serotonergic system, GABAergic system, hdc and hcrt expression in manf deficient embryos. (A and B) 5-HT and (C and D) GABA immunostainingin control MO and manf MOsp morphants in 5 dpf brains. (E) The serotonin level and (F) the epinephrine level measured by HPLC. qPCR analysis of hdc (G) and hcrt (H) transcript levels in 3 dpf larvae (p>0.05, n=3, Student′s t-test). Scale bar=100 μm.
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Reprinted from Developmental Biology, 370(2), Chen, Y.C., Sundvik, M., Rozov, S., Priyadarshini, M., and Panula, P., MANF regulates dopaminergic neuron development in larval zebrafish, 237-249, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.