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Fig. S6
Analysis of Akt Phosphorylation and Interaction between Selected Compounds and eNOS Inhibition, Related to Figure 5 (A) Selected small molecules SST and Gh increased phosphorylation levels of Akt in HUVECs after 8 hr of treatment. (B) Inhibition of PI3K by wortmannin reversed enhancement of HUVEC proliferation by selected small molecules. Wortmannin (0.5 μm) was included as a cotreatment with the selected compounds. Compared with the DMSO control, p = 0.0133 for wortmannin, 0.0003 for SST, 0.0045 for Dip, and 0.0018 for Gh. Compared with SST, p = 0.0021 for SST+wortmannin; Dip, p = 0.0036 for Dip+wortmannin; and Gh, p = 0.0045 for Gh+wortmannin. (C) Inhibition of eNOS reversed enhancement of HUVEC proliferation by selected small molecules. L-NAME (Nω-Nitro-L-arginine [Sigma] dissolved in saline), an eNOS inhibitor, attenuated small-molecule-elicited HUVEC proliferation. Compared with control, p = 4.9E-05 for SST, 0.0139 for Dip, 0.0003 for Gh, 0.0003 for Fh. Compared with SST, p = 5.6E-05 for SST+L-NAME; Dip, p = 0.0456 for Dip+L-NAME; Gh, p = 0.0009 for Gh+L-NAME; and Fh, p = 0.0121 for Fh+L-NAME.