ZFIN Image: Huang et al., 2012, Fig. 3

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Figure Caption

Fig. 3

Flk1-GFP-Labeled Endothelial Cells Respond to Growth Factors and Small Molecules in Primary Culture (A–I) Endothelial cell proliferation and differentiation derived from Flk1-GFP are significantly promoted by 100 ng/ml bFGF (C, bright field, and D) compared with control (A, bright field, and B) at 22 hr after culture. Recombinant VEGF121 (20 ng/ml, F), small molecule SB-431542 (2 μM, G) and the combination of both (H) also remarkably enhance Flk1-GFP endothelial tube formation compared with control (E) at day 5, which exhibits a significant increase in total tube length (I-1, control; I-2, 2 μM SB431542; I-3, 20 ng/ml VEGF121; I-4, 2 μM SB431542 and 20 ng/ml VEGF121). The number values represent total tube length. See also Figure S3.

Acknowledgments

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