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Fig. 1
Life expectancy and colonization of zebrafish swimming larvae infected by different pathogenic bacteria.
A. Life expectancy of axenic zebrafish larvae exposed by bath at 6 dpf to E. coli, E. ictaluri or other pathogenic bacteria. Mean survival is represented by a large hyphen. Standard deviations are also indicated. Asterisks indicate significant difference from non-infected population (*p<0.05, **p<0.01, ***p<0.001). B. Colonization of zebrafish larvae infected by different pathogenic bacteria. CFU counts of axenic zebrafish larvae exposed by bath at 6 dpf to E. ictaluri and other pathogenic bacteria. Mean and standard deviations are indicated. (n = 5). C. Colonization of zebrafish gut monitored at 9 dpf ( = 3 days post infection) by transmitted light microscopy (left) and whole-mount immunohistochemistry using a polyclonal antibody recognizing Gram-negative bacteria (right). Arrows indicate bacterial localization within the gut.