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Fig. S8
The effect of Cxcr7b on Erk1/2 phosphorylation. Western blot analysis showing the level of pErk1/2, total Erk1/2 and α-tubulin (loading control) in whole embryo lysates at 10-11 hpf. (A) The levels of Erk1/2 and its phosphorylated form are not altered by knocking down Cxcr7b (cxcr7b MO, compared with control MO), nor by overexpressing the protein (cxcr7b RNA, compared with gfp RNA). (B) The level of phosphorylated Erk1/2 is significantly reduced by treatment with 100 μM Mek1/2 inhibitor U0126.
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