ZFIN Image: Yano et al., 2012, Fig. S8

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Figure Caption

Fig. S8

Pectoral fin formation in HG21C line (tcf7 mutant fish). (A-F) Whole-mount in situ hybridization of pectoral fin buds of heterozygous (control) and homozygous embryos. In heterozygous embryos at 34 hpf, fin buds were formed, and tcf7 (A) and fgf24 (B) were expressed in the AER/AF, and fgf10a (C) was expressed within the fin mesenchyme. In homozygous embryos of HG21C, tcf7 mRNA was not detected (D), but fin buds were formed normally with mRNA expression of fgf24 (E) and fgf10a (F). Genotype analyses of the HG21C mutant by genome PCR. In heterozygous embryos, 1119-bp fragments were detected (insets in A-C) but these were not in homozygous embryos (insets in D-F).

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