Six3b is required for specification of ventral telencephalon. (A,B) nkx2.1b expression in telencephalon (arrows) of control (A) and six3b;six7-deficient (B) embryos at the 16-somite stage. (C,D) emx3 expression in control (C) and six3b;six7-deficient (D) embryos at the 12-somite stage. (E,F) nkx2.1b (purple) expression in telencephalon (arrows) and ectopic expression (black arrowheads) at 24 hpf in Tg(hsp70l:Gal4-VP16); Tg(UAS:six3b) embryos globally misexpressing six3b (red) (F) compared with embryos not subjected to heat shock (E). GOF denotes gain of function. Embryos are shown in lateral view with anterior towards the left. Red and green arrowheads indicate dorsal and ventral edges of the telencephalon, respectively. Scale bars: 100 μm
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