Progeny of SPCs contributes to the terminal neuromasts. (Aa,Ab) Primordium region of 18s embryo injected with Kaede mRNA, before (a) and after (b) photoactivation in the most caudal SPC cluster. The main primordium and SPCs are outlined with dashed lines. (Ac) Low magnification of the same embryo at prim-11, showing red cells (white arrowhead) in the migrating primordium. No deposited red cell is observed between the region of activation (red arrowhead) and the primordium. (Ad) High magnification of the primordium shown in c, showing red cells in the leading part of the primordium. (Ae) Low magnification of the trunk at 45h, showing red cells (white arrowheads) in the last deposited neuromast and in the primordium reaching the tail. No red cell is deposited more anteriorly. (Af) High magnification of the primordium and the terminal neuromast shown in e. See also supplementary material Fig. S4. (Bg-Bj) Examples of confocal sections showing the primordium region of cldnb:lyngfp embryos at 22s, 2 hours after partial laser ablation of SPCs. H2B-RFP-labelled fragmented DNA of dying SPCs is indicated by white arrows. (Bk) Phenotype observed at 48h in embryos in which a subset of SPCs were laser-ablated on the left side. The primordium is small and unable to complete its journey, compared with the control non-ablated side. The inset shows a high magnification of the arrested primordium. mp, main primordium; nt, neural tube; op, otic placode; s, somite. Scale bars: 25 μm.
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