IMAGE

Fig. S9

ID
ZDB-IMAGE-120329-30
Source
Figures for Hozumi et al., 2012
Image
Figure Caption

Fig. S9

Pre-mRNA splicing of the housekeeping gene actb1, but not b2m, is unaffected in the Ddx46hi2137/hi2137 mutant. (A–D) Scheme of the b2m and actb1 pre-mRNA regions analyzed for splicing (boxes, exons; lines, introns; arrows, primers) (A, C). The splicing status of b2m and actb1 pre-mRNA was monitored using RT-PCR with the primers indicated in scheme A and C, respectively. Total RNA was isolated from the heads of Ddx46hi2137/hi2137 mutants (mut) and control (con) larvae. Unspliced b2m mRNAs were retained in the Ddx46hi2137/hi2137 mutants compared to the control larvae (arrowheads in B), whereas the splicing of actb1 was unaffected in the Ddx46hi2137/hi2137 mutants (arrowheads in D). Unspliced and spliced PCR products were verified by sequencing. +RT refers to the validation reaction itself, and -RT represents the respective control reaction without reverse transcriptase. 18S rRNA was used as a loading control. M, DNA size markers (sizes in bp). Control larvae were sibling WT or Ddx46hi2137/+ larvae and had normal phenotypes.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One