ZFIN Image: Hozumi et al., 2012, Fig. 2

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Figure Caption

Fig. 2

Identification of the mor gene and analysis of the hi2137 allele.

(A) Meiotic and physical map schematic of the mor locus on chromosome 21. The number of recombinants and larvae genotyped is shown for each microsatellite marker. (B) Sequencing cDNA from WT and mor^ha4 larvae revealed a nucleotide exchange from T to G, which resulted in an Ile-to-Ser transition at amino acid 942 in the mor^ha4 mutant. (C) Genomic structure of the Ddx46 gene showing the viral insertion site in the hi2137 allele (red). Exons are boxes, with coding and non-coding sequences in blue and green, respectively. The viral insertion (red arrow) occurs in the first intron between exons 1 and 2. (D) Northern blot analysis of Ddx46^{hi2137/hi2137} mutants and control larvae at 3.5 dpf. No Ddx46 transcript was found in the Ddx46^{hi2137/hi2137} mutants, whereas the level of actb1 transcript in the mutants was the same as that in control larvae. Control larvae were sibling WT or Ddx46^hi2137/+ larvae and had normal phenotypes.

Figure Data

Expression / Labeling details

Acknowledgments

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