ZFIN Image: Liao et al., 2012, Fig. S2

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Figure Caption

Fig. S2 Gene trap lines appais22Gt and aplp2is23Gt contain single Tol2 transposon integrations. A: Genomic Southern blot hybridized with red fluorescent protein (RFP) probe. DNA from wild-type (1) and homozygous appais22Gt/is22Gt adults (2) digested with BglII; wild-type (3) and homozygous aplp2is23Gt/is23Gt adults (4) digested with BamHI. As predicted based on the location of the Tol2 transposon integration site in the appa gene, a single BglII restriction fragment of <2.8 kb (arrow) is present in the appais22Gt/is22Gt adult. The faint higher band is most likely due to incomplete digestion (see B, repeat of BglII digestion). In BamHI digested DNA from the aplp2is23Gt/is23Gt adult, a single restriction fragment of <7.9 kb (arrowhead) is detected. This is the expected size based on the predicted integration site of the Tol2 transposon in the aplp2 gene in line aplp2is23Gt. B: To confirm the presence of a single Tol2 transposon integration in the appa gene in line appais22Gt, genomic DNA from wild-type, heterozygous appais22Gt/+, and homozygous appais22Gt/is22Gt adults was digested with BglII or EcoRV, blotted, and hybridized with the RFP-specific probe. A single restriction fragment of the expected size (2.8 kb BglII; 8.9 kb EcoRV) is detected in heterozygous and homozygous appais22Gt adults (arrow and arrowhead).

Acknowledgments

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