ZFIN Image: Dohn et al., 2012, Fig. S2

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Figure Caption

Fig. S2

Dkk and Wnt8 expression from transgenic lines at the 16 s stage is able to affect the expression of axin2. Axin2 is a Wnt responsive gene (Weidinger et al., 2005). (A,C) HCSEs at 20 s. (B,D) GFP + Tg(hsp70l:dkk1-GFP)^w3 and Tg(hsp70l:wnt8a-GFP)^w34 embryos at 20 s, respectively. (B) After decreasing Wnt signaling at the 16 s, axin2 expression is significantly downregulated relative to HCSEs (A). Arrowheads in A and B indicate the midbrain hindbrain boundary and the tip of the tail, where axin2 expression is significantly downregulated relative to HCSEs. (D) After increasing Wnt signaling at the 16 s, axin2 expression is significantly upregulated compared to HCSEs (C). Arrowheads in C and D indicate the anterior brain and spinal cord, where axin2 is upregulated relative to HCSEs. Views are lateral with anterior left.

Acknowledgments

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Reprinted from Developmental Biology, 361(2), Dohn, T.E., and Waxman, J.S., Distinct phases of Wnt/β-catenin signaling direct cardiomyocyte formation in zebrafish, 364-76, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.