IMAGE

Fig. S1

ID
ZDB-IMAGE-120131-25
Source
Figures for Caron et al., 2012
Image
Figure Caption

Fig. S1 Generation of functional Tg(hsp:dkk1-GFP) and Tg(hsp:β-catenin-GFP) transgenic fish. (A) Schematic of hsp70 promoter-driven dkk1 expression. An upstream sequence (<1.6 kb) of zebrafish hsp70-4 (Halloran et al., 2000) and full-length zebrafish dkk1 encoding sequence were inserted in-frame into the tol2 GFP vector. The resulting plasmid was co-injected with tol2 transposase RNA into one-cell staged embryos. (B) The fused GFP tag allows discrimination of Dkk1-expressing embryos (GFP+) from non-transgenic siblings (GFP-) after heat-shock treatment. Transgenic Dkk1 embryos that were heat activated at 30% epiboly had tail/trunk truncation, a characteristic phenotype of loss of Wnt signaling (Lekven et al., 2001). Heat-shocked non-transgenic siblings exhibited normal morphology. (C) Representative images of pitx2 expression in transgenic Dkk1 embryos (Dkk+) heat-shocked at the bud stage. Shown are dorsal views of 22-somite staged embryos. Normal left-sided expression became randomized. (D) Schematic of hsp70 promoter-driven β-catenin1 expression. An upstream sequence (<1.6 kb) of zebrafish hsp70-4 (Halloran et al., 2000) and full-length zebrafish β-catenin1 encoding sequence were inserted in-frame into the tol2 GFP vector. (E) Transgenic β-catenin1 embryos activated at the sphere stage had anterior head truncation, a characteristic phenotype of gain of Wnt signaling (Lekven et al., 2001). (F) Induction of β-catenin1 (Catenin+) at the sphere stage abolished the expression of forebrain marker foxg1 but maintained the expression of midbrain-hindbrain boundary marker her5 compared with heat-shocked non-transgenic siblings (Catenin-). Arrows indicate foxg1 expression.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development