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Fig. 3 NCPC domains when BMP signaling is reduced in somitabun and snailhouse and increased in swirl embryos.
foxd3 expression at the end of gastrulation in tBR mRNA injected embryos of sbn (A) and of snh (B), and smad5 mRNA injected into swr embryos (C). (A) Injection of tBR mRNA into sbn mutants leads to the majority of embryos displaying a “swr” phenotype. (B) Injection of a low 15 pg dose of tBR mRNA into snh mutants leads to nearly equal numbers of “snh” and “sbn” phenotypes. Injection of a higher 100 pg dose leads to the majority of embryos displaying the “sbn” phenotype and also a percentage displaying a stronger “swr” phenotype. (C) Injection of a low 30 pg dose of murine smad5 mRNA results in nearly half of embryos displaying a “sbn” phenotype. Injection of a higher 150 pg dose results in a small percentage of embryos displaying the “swr” phenotype, and the rest of the embryos divided between “sbn”, “snh”, and WT phenotypes.