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Fig. 2 V0v, V1 and V2 cells persist in the absence of Hh signalling. (A-I3) In situ hybridisation for the indicated marker genes in lateral views of 24-hpf trunk of WT, smo mutant and MZsmo mutant zebrafish embryos. Asterisks indicate tal2-expressing cells (B′,B′′) and islet2a-expressing MNs (C′,C′′) that still form in smo and MZsmo mutants. islet2a is also expressed in more dorsal Rohon-Beard cells (arrowheads in C-C′′). Rohon-Beard cells are unaffected in smo or MZsmo mutants (C′,C′′). eng1b is also expressed in muscle pioneer cells ventral to the spinal cord in WT embryos (arrowheads in G). These cells are absent in smo or MZsmo mutants (G′,G′′). Scale bar: 50 μm.