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Fig. S5
Residual early-born olfactory neurons fail to express olfm1a and gef in neurog1 mutant olfactory placodes. (A-L) Confocal sections of in situ hybridisation and immunolabelling against olfactomedin1b (olfm1b, A-F) or gefiltin (gef, G-L) and GFP in wild-type (A-C,G-I) and neurog1 mutant embryos (D-F,J-L) in the Tg(8.4neurog1:GFP) background at 24 hpf. Whereas early-born olfactory neurons differentiate in neurog1 mutant embryos, they do not express these markers of specification. Placodes are oriented with anterior up and lateral to the right. Scale bars: 10 μM.
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