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Fig. S9 Specificity of Frzb morpholinos. (A) Position of antisense morpholinos designed to block Frzb translation on the sequence of 52-UTR and the 52 part of Frzb ORF. Injection of 180 pg of Frzb-GFP mRNA at the one-cell stage results in (B) embryos expressing GFP at the late blastula stage. Coinjection of Frzb-GFP mRNAs with 8 ng of morpholino Frzb_1 (C) or Frzb_2 (D) prevents translation of the Frzb-GFP fusion protein. (Left) Bright field. (Right) Visualization of GFP fluorescence.
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