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Fig. S2
Complete repression of β-catenin signaling in ich embryos induces neuroectoderm with correct AP pattern. Untreated ich embryos do not express emx1, krox20, val, or hoxb6b (B,E,H), while their siblings coinjected with βcatMOs (C,C′,F,F′,I,I′) express these neuroectodermal markers in a correct order at 22 hpf, with the anterior to posterior direction corresponding to proximal to distal relative to the yolk (compare A with C,C′, D with F, F′, and G with I,I′). The following probe-pairs were used: emx1 (blue) and krox20 (red) (A-C′), krox20 (red) and val (blue) (D-F′), and krox20 (red) and hoxb6b (blue) (G-I′).