|
Fig. S1
Wnt-, TGFβ- and FGF-signaling have pivotal roles in the posteriorization of the neuroectoderm of embryos both inhibited in BMP signaling and devoid of organizer activity. Single in situ hybridization with otx1 (A,D,G,J,M,P,S,V,Y) or gbx1 (B,E,H,K,Q,T,W,Z) probes or double in situ hybridization with both probes (C,F,I,L,O,R,U,X,A′) is shown for wild-type embryos (A-C), untreated ich embryos (D-F), or ich embryos treated with bmp2bMO (G-I), the two β-cat MOs (J-L), bmp2bMO plus the two β-cat MOs (M-O), or ich embryos injected with antivin mRNA (P-R), antivin mRNA plus bmp2bMO (S-U), SU5402 (V-X), or SU5402 plus bmp2bMO (Y-A′). Wild-type embryos [A-C] are shown in dorsal views, while ich embryos are shown in lateral views. All embryos are at ~100% epiboly. Arrowheads in the antivin (atv) treated embryos point to the edge of the germ-ring.