ZFIN Image: Varga et al., 2011, Fig. 1

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Figure Caption

Fig. 1

Two independent ways of inhibiting BMP signaling result in massive ectopic expression of chordin. (A-E) BMP signaling detected by immunohistochemistry using an anti phospho-Smad1/5/8 antibody; green dots indicate nuclei containing the phosphorylated Smad. (F-J) In situ hybridization with chd probe. (K-O) Expression of the epidermal marker p63. Wild-type embryos (A,F,K) are shown with in lateral view with ventral side to the left. Untreated ich embryos (B,G,L), and ich embryos injected with bmp2bMO (C,H,M), or with the two βcatMOs (D,I,N), or with all three MOs (E,I,O) are shown in lateral views. All embryos are at ~70% epiboly.

Acknowledgments

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