ZFIN Image: Kikuchi et al., 2011, Fig. 1

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Figure Caption

Fig. 1

Resection of the Ventricular Apex Stimulates Immediate, Organ-Wide Morphological and Molecular Changes in Endocardium

(A–D) Transmission electron microscope (TEM) analyses of endocardium in uninjured (A) and injured ventricles (B–D). Arrowheads, endocardial nuclei; arrows, endocardial cell bodies; M, cardiac muscle; asterisk, red blood cell. Scale bar represents 2 μm.

(E–K) raldh2 expression assessed by in situ hybridization (E–J) and Raldh2 immunostaining (K) in uninjured (E) and injured (F–K) ventricles. Brackets in (I–K), injury site. Arrows in (J and K), epicardial cells. Scale bar represents 100 μm (E–Q).

(L–N) Confocal images of altered endocardial cell shape, and enhanced flk1 driven DsRed2 fluorescence in the injury site (L, brackets) in a 7 dpa cmlc2:EGFP; flk1:DsRed2 double transgenic ventricle. Arrowheads, endocardial nuclei; arrows, endocardial lining of myofiber. An antibody against DsRed was used. DAPI (42-6-Diamidino-2-phenylindole) stains nuclei.

(O–Q) Sections of 7 dpa fli1:EGFP (O), hand2:EGFP (P), or gata5:EGFP (Q) transgenic ventricles. Brackets, injury sites. Arrowheads in inset, Raldh2⁺/EGFP⁺ endocardial nuclei with rounded morpholog

Acknowledgments

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Reprinted from Developmental Cell, 20(3), Kikuchi, K., Holdway, J.E., Major, R.J., Blum, N., Dahn, R.D., Begemann, G., and Poss, K.D., Retinoic Acid production by endocardium and epicardium is an injury response essential for zebrafish heart regeneration, 397-404, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell