Fig. S3

Fig. S3

Puma and bax-a morpholino efficacy. Schematic of puma genomic locus, splicing pattern and observed spliced and unspliced products (A). RT-PCR (B) using primers to detect spliced (primers F + R2, SPLICED) and unspliced (primers F + R1, UNSPLICED) puma transcripts was performed on control MO (Ct) and puma SB MO (Pu)-injected embryos (0.56 pmol each). Loss of spliced puma (SPLICED, compare Ct and Pu) and increase in unspliced puma (UNSPLICED, compare Ct and Pu) upon puma SB MO injection was observed. PCR using primers to EF1a confirmed near-equivalent amounts of cDNA were used. Puma splice-blocking and bax-a morpholinos protect against gamma irradiation induced apoptosis (C). Control MO-injected embryos (C, Ctrl MO) show extensive Caspase3 activation (anti-active Caspase3, green) after exposure to 12.5 Gray irradiation, while both pumaSB and bax-a MO-injected embryos show much reduced levels. Embryos were irradiated at 18ss, and subsequently grown for 3 h at 28.5 °C.