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Fig. 1 Mesendoderm induction by Mxtx2. (A-X) Control gfp mRNA (50 pg) or mxtx2 mRNA (10 pg) was injected into whole zebrafish embryos (A-L), or Ctrl MO (8 ng) or Mxtx2 MO (2 ng) was injected into the yolk syncytial layer (YSL) (M-X), and embryos were fixed at 30% epiboly (4.7 hpf) and stained by whole-mount in situ hybridization (WISH) for the indicated markers. Animal pole views are shown. Fractions indicate the number of equivalent outcomes/number of embryos observed. See also Fig. S1 in the supplementary material.
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