ZFIN Image: Chung et al., 2010, Fig. S8

Image

Figure Caption

Fig. S8 Mosaic knockdown of alk8 cell-autonomously generates ventral bud-derived β-cells. (A) Schematic diagram of the cell transplantation protocol. Tg (ins:GFP);Tg(ins:dsRed) donors were injected with cas/sox32 mRNA and alk8 splice-blocking MO along with Alexa Fluor 647 dextran (blue), and the donor cells were transplanted into Tg(ins:GFP);Tg(ins:dsRed) hosts. (B–C′) Confocal projections of Tg(ins:GFP)- and Tg(ins:dsRed)-expressing islets containing control (B and B′) and alk8 morphant (C–D′) donor cells (blue) at 60 hpf. Control donor cells incorporated into Tg(ins:GFP)- and/or Tg(ins:dsRed)-expressing β-cells without showing any preference. In contrast, alk8 morphant donor cells (blue) gave rise to many GFP-only–positive β-cells (arrows). For clarity, the green color is excluded in the corresponding Left panels (B–D).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA