ZFIN Image: Chung et al., 2010, Fig. S5

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Figure Caption

Fig. S5 alk8 splice-blocking morpholino-injected donor cells behave like control cells and can give rise to dorsal bud-derived endocrine cells. (A) Schematic diagram of the cell transplantation protocol. Tg(ins:GFP) donors were injected with cas/sox32 mRNA and alk8 MO along with rhodamine dextran, and the cells were transplanted into Tg(ins:GFP) hosts. (B and C) Ventral confocal images of Tg(ins:GFP) (green), β-catenin (white), and rhodamine dextran (red) at 18 hpf (the notochord is outlined by yellow dashed lines). The donor cells (red) did not give rise to ectopic Tg(ins:GFP)-expressing cells, because maternally derived Alk8 is present during early stages.

Acknowledgments

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