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Fig. S17 Multicolor imaging of GalNAz-labeled glycans at the cleavage furrow of dividing cells. Zebrafish embryos were microinjected with GalNAz or no sugar and allowed to develop to 10 hpf, at which point they were reacted with DIFO-555 (100 μM, 1 h). Immediately following this reaction, the embryos were reacted with DIFO-488 (100 μM, 15 min) and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images. Green, DIFO-488; red, DIFO-555. Scale bars: 100 μm (A), 10 μm (B).
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