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Fig. S14 Simultaneous visualization of O-glycans and sialylated glycans during the early segmentation period. Zebrafish embryos were microinjected with GalNAz or no sugar, allowed to develop to 10 hpf, and then bathed in NaIO4 (500 μM, 30 min) or no reagent. Embryos were then reacted in a mixture of DIFO-555 (100 μM) and AO-488 (100 μM) in PBS (pH 6.7) for 1 h and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, AO-488; red, DIFO-555. Scale bars: 200 μm (A), 20 μm (B).
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Full text @ Proc. Natl. Acad. Sci. USA