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Fig. S12 Dual labeling of sialic acids with ManNAz and NaIO4 followed by reaction with DIFO- and aminooxy-fluorophores shows substantial colocalization. Zebrafish embryos were bathed in 5 mM Ac4ManNAz or no sugar for three days, then treated with NaIO4 (500 &*mu;M, 30 min) or no reagent. Embryos were then reacted in a mixture of DIFO-555 (100 μM) and AO-488 (100 μM) in PBS (pH 6.7) for 1 h and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images. Green, AO-488; red, DIFO-555. Scale bar: 10 μm.
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