Image
|
Figure Caption
Fig. 3
Imaging of sialylated glycans using a nonmetabolic approach. (A) Schematic for chemical labeling of sialylated glycans by treatment with NaIO4 to expose aldehydes on sialic acids, followed by detection using aminooxy-fluorophore conjugates. (B and C) Zebrafish embryos (10 hpf) were bathed in NaIO4 (500 μM, 30 min, top row) or no reagent (bottom row), reacted with aminooxy-Alexa Fluor 488 (AO-488, 100 μM, 1 h, pH 6.7), and imaged by confocal microscopy. (B, left panel) Brightfield; (B, right panel) and (C), maximum intensity z-projection images of AO-488 fluorescence. [Scale bars: 200 μm (B), 20 μm (C).]
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Proc. Natl. Acad. Sci. USA