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Fig. 1 MZfz7a/b Double Mutants Exhibit Reduced CE Movements and Reduced Stereotypical Germ-Layer Progenitor Cell Division Orientation
(A and G) Differential interference contrast (DIC) images of a wild-type (A) and MZfz7a/b (G) embryo at bud stage (10 hpf). Arrowheads demarcate body axis.
(B, C, H, and I) Expression pattern of no tail (ntl), hatching gland gene 1(hgg1), and distal-less homeobox gene 3 (dlx3) marking the notochord, prechordal plate, and anterior neural plate, respectively, in a wild-type (B and C) and a MZfz7a/b (H and I) embryo at bud stage. Animal (B and H) and dorsal (C and I) views.
(D–F and J–L) Frequency distribution of pairs of division-orientation angles (θ, φ) in wild-type (D–F) and MZfz7a/b (J–L) embryos during gastrulation (6–10 hpf). Chi-square test on the combined angles: wild-type versus MZfz7a/b (p < 0.05).
(D and J) Distribution frequency of pairs of division-orientation angles in wild-type (D) and MZfz7a/b (J).
(E and K) Distribution of azimuthal angles (θ) in wild-type (E) and MZfz7a/b (K).
(F and L) Distribution of elevation angles (φ) in wild-type (F) and MZfz7a/b (L).
(M) Schematic dorsal view of a zebrafish embryo at the early gastrula stage. The dotted line demarcates the shield. Pairs of daughter cells are shown as wired gray spheres. The reference coordinate system used to calculate the angles of the cell divisions is indicated by the red (antero-posterior), green (dorso-ventral), and blue (left-right) axes.
(N) Picture of the dorsal side of a wild-type embryo at 60% epiboly. Two daughter cells resulting from one representative division are marked by red spheres.
The same coordinate system as in (M) is used. The scale bar represents 40 μm.
(O) Schematic diagram showing the azimuthal (θ) and the elevation (φ) angles for a single cell division represented by wired gray spheres and red nuclei. The embryonic axes are shown in respect to the reference system.
See also Figures S1 and S2 and Table S1.