IMAGE

Fig. 5

ID
ZDB-IMAGE-100903-54
Genes
Source
Figures for Wang et al., 2010
Image
Figure Caption

Fig. 5 Moesin1 is required in the endothelial cells for tubulogenesis. (A-F) The Tg(flk1:moesin1-egfp) line partially rescues the Moesin1 knockdown phenotype. (A,B) A Tg(flk1:moesin1-egfp) zebrafish embryo injected with 4 ng control MO. All the ISVs were perfused with tetramethylrhodamine-dextran (TMRD, red). (C,D) Wild-type sibling embryo injected with 4 ng moesin1 MO. Most ISVs are not perfused with TMRD, although circulation of TMRD is observed in the axial vessels. (E,F) A Tg(flk1:moesin1-egfp) embryo injected with 4 ng moesin1 MO. Most ISVs are perfused with TMRD. (G-I) Same experiment as in A-F in Tg(flk1:moesin1-egfp)/Tg(flk1:nlsmCherry) embryos shows that the endothelial cells are present in the ISVs despite a lack of circulation at 54 hpf. (J-O) Transplantation of endothelial cells was used to determine autonomous verses non-autonomous effects. (J-L) Normal endothelial tubulogenesis occurs when the donor embryo was injected with control MO. (M-O) Transplanted endothelial cells from Moesin1 knockdown embryos fail to undergo normal tubulogenesis (arrowheads), whereas the ISV in the recipient embryo (arrow) completes lumen formation.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development