ZFIN Image: Rojas-Muñoz et al., 2009, Fig. 7

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Figure Caption

Fig. 7

Initial events of wound healing occur in ErbB3 mutants. (A and B) In wild-type adults, caudal fin regeneration is completed around 10 days post-injury (dpi). (C and C′) Conversely, homozygous mutants for the ErbB3 allele hps^t21411 do not regenerate properly even after 30 dpi. (D) At the histological level, closure of the wound epidermis is not affected in homozygous mutants for hps^t21411 at 1 dpi compared to wild-type controls although the formation of the blastema is delayed. At 3 dpi, the blastema in homozygous mutants is smaller compared to wild-type controls. Additionally, the distal blastema is absent in some individuals (arrowhead on inset). All sections are longitudinal. Scale bar 50 μm. (E) Polymorphonuclear leukocytes (PMNs) migrate to the wound site in wt animals, ErbB3 mutants and wt animals incubated with ErbB/PI3K (PD/LY) inhibitors. The initial site of DiI injection and the location of putative PMNs at 24 hpi is demarcated with white and red arrowheads, respectively. Most labeled cells migrate towards the amputation plane.

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Acknowledgments

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Reprinted from Developmental Biology, 327(1), Rojas-Muñoz, A., Rajadhyksha, S., Gilmour, D., van Bebber, F., Antos, C., Rodríguez Esteban, C., Nüsslein-Volhard, C., and Izpisúa Belmonte, J.C., ErbB2 and ErbB3 regulate amputation-induced proliferation and migration during vertebrate regeneration, 177-190, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.