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Fig. 5 Wnt/β-catenin signaling is not involved in the initiation of vsx2 and rx1 expression but affects their maintenance. A,B: At 32 hours postfertilization (hpf), rx1 is broadly expressed in wild-type and apc mutant eyes, even though Wnt/β-catenin target genes are already expanded at this stage. C,D: In 32 hpf wild-type eyes, vsx2 is more strongly expressed in all cells of the dorsal, whereas in apc mutant eyes, it is also present in the ventral domain (D). E: In 48 hpf wild-type eyes, rx1 is expressed in all peripheral cells, encompassing the otx1, sox2, and atoh7 domain, as well as differentiating photoreceptor cells. G: In 48 hpf wild-type eyes, vsx2 is expressed in an overlapping domain with the exception of photoreceptor cells. F,H: In 48 hpf apc mutant cells, rx1 and vsx2 are expressed in more central cells but are down-regulated in the expanded CMZ.