ZFIN Image: Okuda et al., 2010, Fig. S4

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Figure Caption

Fig. S4 Effects of the B1 sox QKD revealed using transgenic lines with the GAL4-medeated reporter expression in the CNS and axial mesoderm. (A) Effects of QKD on CNS development were examined using the GAL4 enhancer trap line, which reports the activity of nr2f2 (couptfβ) neural enhancers. Double transgenic embryos harboring GAL4VPmad2 (#5m-3) and UAS:DsRedExDR normally show reporter expression in the CNS, which mimics neural nr2f2 expression. Injection of the MOs for QKD abolished this reporter expression, indicating impairment of CNS development. (B) Effects of QKD on axial mesoderm development, examined using the GAL4VP16(hg/nc) line. Double transgenic embryos harboring the GAL4VP16(hg/nc) transgene and UAS:DsRedEx show reporter expression in the hatching gland (hg) and notochord (nc). Strong expression of UAS:DsRedEx was observed after 1 dpf in this line. Injection of the MOs for QKD did not reduce reporter expression, indicating normal axial mesoderm differentiation in the morphants. However, hatching gland cells remained as a single ball-like structure in the heads of the severe morphants (arrowhead).

Acknowledgments

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